Online Teaching Materials

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Antimicrobial Silver

In this lab, students will make a silver colloidal mixture. Silver solution will be reduced and stabilized with sodium citrate in a boiling water bath. The colloidal dispersions formed will be poured into Petri dishes, soaked up into filters, and placed on top of a bacterial agar plate. The silver colloid should be yellow in color. Silver colloidal particles are 20-50 nm in size. If the silver nanoparticles prevent bacterial growth, there should be a ring on inhibition around where the filters soaked with nanoparticles are placed on the bacterial agar plate.

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